Even though Real were 1-0 behind [leading 3-1 on aggregate] midway through the first half, the Spanish defender appeared to be in relaxed mood.
Gianluigi Buffon was infuriated by referee Michael Oliver's decision to award Madrid a penalty in the 93rd minute and was sent off for his aggressive reaction but Marcelo insisted there was no doubt over the spot-kick.
Real Madrid fans in Ghana joined thousands of the club supporters around the globe to celebrate the team's triumph over Juventus which sealed a semi-finals berth in the UEFA Champions League. The latter were eliminated in surprise circumstances on Tuesday, surrendering a three-goal lead against Roma.
Juventus came out desperate for the opener in a pulsating opening 45 minutes, and got the goal they craved after just two minutes as Mandzukic headed home at the far post from Sami Khedira's deft cross.
This was reminiscent of Madrid's Dortmund defeat en route to the 2014 Champions League crown, when Carlo Ancelotti's side lost 2-0 in Germany after winning the first match 3-0 in Madrid.
The closest Real Madrid came to scoring in the first half was a shot in the final seconds by Rafael Varane that hit the cross-bar. Substitutes: Lucas Vasquez (for Casemiro, 45) 6, Marco Asensio (for Bale, 45) 6, Mateo Kovacic (for Modric, 75) 6.
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The home side continued to struggle on offense and when they did breach the Bianconeri wall, Buffon was always there to dispel the danger, making an outstanding stop against Ronaldo.
Gianluigi Buffon, Juventus goalkeeper: "To award such a doubtful, or super doubtful penalty, just ahead of the final whistle and destroy the work of a team who gave absolutely everything, you have to have a trash bin instead of your heart".
Ronaldo set another new record, becoming the first to score in 11 straight Champions League matches.
"We are in the semi-final now and I'd rather talk about the game".
"Pulses were racing, but I calmed myself down".
"I don't understand the protest by Juventus", Ronaldo told reporters after the game.
Needless to say, the reaction to the match has polarised opinion.